《植物生理学报》 2009, 45(3): 233-238

番茄转化酶抑制子基因克隆及其表达分析

姜晶*, 李冬妮, 孙威

沈阳农业大学园艺学院, 辽宁省设施园艺重点实验室, 沈阳110161

通信作者：姜晶；E-mail: jj_syau@hotmail.com；Tel: 024-88487143

摘　要：

从普通栽培型番茄品种 ‘ 桃太郎’、野生醋栗番茄和潘那利番茄的幼苗中采用RT-PCR 方法, 扩增出转化酶抑制子基 因 cDNA 序列的部分片段。经测序表明: 不同基因型番茄的转化酶抑制子基因同源性高达 97.97% 以上。采用半定量 RT- PCR 方法对 ‘ 桃太郎 ’ 苗期根、茎、叶和花后功能叶、花期子房以及果实不同发育阶段不同部位的表达进行检测, 结果表 明: INH 在根中表达较弱, 茎中有强烈表达, 从幼叶到衰老叶表达逐渐减弱; 同一时期INH在果肉中表达相对较强, 维管束次 之, 胶质胎座相对较弱; 花期子房中 INH 的表达逐渐增强, 花后3 d 左右达到最大, 花后5~8 d 表达量迅速下降。

关键词：番茄; 转化酶抑制子; RT-PCR; 基因表达

收稿：2008-11-06   修定：2009-01-14

资助：辽宁省教育厅科学研究技术计划(05L412)。

Cloning and Expression Analysis of Invertase Inhibitor Gene (INH) from Tomato (Lycopersicon esculentum Mill.)

JIANG Jing*, LI Dong-Ni, SUN Wei

College of Horticulture, Shenyang Agricultural University, Liaoning Key Laboratory of Protected Horticulture, Shenyang 110161,China

Corresponding author: JIANG Jing; E-mail: jj_syau@hotmail.com; Tel: 024-88487143

Abstract:

The cDNA fragments of invertase inhibitor were obtained by RT-PCR from different genotype tomato ‘Momotaro’, Lycopersicon pimpinellifolium and Lycopersicon pennellii. These sequences showed highly homology (above 97.97%) to the published sequences of INH respectively. The expression patterns of the cDNAs were examined in ‘Momotaro’ various tissues during different stages by semi-quantitative RT-PCR. The results showed that INH gene expressed lowly in roots, significantly highly in stem and decreased from young leaves to senescent leaves. The expression of INH gene in sarcocarp was the highest relatively, which in fruit vascular was secondary and in pectinic placenta was weak. The expression of INH gene increased in ovaries of inflorescence stage, and was highest on the 3rd day after anthesis, then rapidly decreased from 5 to 8 days after anthesis.

Key words: tomato (Lycopersicon esculentum); invertase inhibitor; RT-PCR; gene expression